【摘要】 目的从云南传统发酵豆豉中分离乳酸菌,并从中筛选具有高抑菌活性的菌株,进一步研究其抑菌谱,并初步分析其抑菌机制,为新型生物防腐剂的开发提供理论依据。方法应用传统纯培养技术及spot-on-lawn法从云南传统发酵豆豉中筛选得到具有抑菌作用的乳酸菌40株,并从中筛选得到1株具有高效抑菌活性的植物乳杆菌YM-4-3菌株。通过HPLC和紫外分光对其主要抑菌物质进行分析与抑菌试验。结果 YM-4-3菌株是1株具有新型生物防腐剂应用潜力的乳酸菌菌株。结论初步推测YM-4-3菌株抑菌机制为有机酸主导,细菌素和其他化学抑菌物质协同作用。更多还原

【Abstract】 Objective To screen Lactic acid bacteria strains with high inhibitory activity from Yunnan Douchi,analyze its mechanism of bacteria inhibition.Method Lactic acid bacteria strains were isolated from Yunnan traditional fermented Douchi using traditional techniques of pure culture.Their antibacterial activity were assayed by spot-on-lawn method.Forty strains were identified as Lactic acid bacteria.YM-4-3 strain was identified as Lactobacillus plantarum with high antibacterial activity.Analysis and antibacterial experiments were performed on the major inhibitory substances with HPLC and ultraviolet spectrophotometric methods.Result The strain YM-4-3 showed a wide antibacterial spectrum with ability to inhibit both Gram pasitive and negative bacteria.Conclusion It was preliminarily conjectured that the mechanism of bacteriostatic was organic acid as leader,then bacteriocin and other chemistry substances against them.更多还原