【摘要】 目的探讨不同比活度的¹³¹I标记大肠癌单抗片段对大肠癌细胞结合率的影响。方法应用37MBq的¹³¹I分别标记1000、200、100、50、25μg的单抗片段,测定标记率>95%后与2×10⁶的大肠癌细胞反应30min,离心、洗涤2次后测量结合率;对照组设骨髓瘤细胞,观察非特异性结合率;选取结合率最高组,增加细胞数,观察结合率变化。结果 1000、200、100、50、25μg的¹³¹I大肠癌单抗片段的放射性比活度分别为37、185、370、740、1480kBq/μg;与2×10⁶大肠癌细胞的结合率分别为10.1%、14.7%、35.1%、32.7%、22.6%;非特异性结合率为0.8%;增加细胞数可以进一步增加结合率。结论比活度为370⁷40 kBq/μg的碘标记大肠癌单抗片段对大肠癌细胞有较高的结合率。更多还原

【Abstract】 Objective To explore effects of different specific activities of anti-colorectal carcinoma monoclonal antibody fragment labeled by ¹³¹I on binding rate of colorectal carcinoma cells.Methods The 37 MBq ¹³¹I was adopted to label 1000,200,100,50 and 25μg monoclonal antibody fragment respectively,which reacted with colorectal carcinoma cells for 30 mins after the labeling rate was determined to be greater than 95%.Binding rate was measured after centrifugation and washing for 2 times.The control group of myeloma cells was set up to observe non-specific binding rate.In a group with the highest binding rate,cells were increased to observe the binding rate.Results The specific activity of anti-colorectal carcinoma monoclonal antibody fragment labeled by 1000,200,100,50 and 25μg ¹³¹I was 37,185,370,740 and 1480kBq/μg respectively;the binding rate with 2×10⁶ colorectal carcinoma cells was 10.1%,14.7%,35.1%,32.7%and 22.6%respectively;and the non-specific binding rate was 0.8%.Binding rates could be further improved by increasing the cell number.Conclusion Anti-colorectal carcinoma monoclonal antibody fragment with specific activity of 370-740 kBq/μg labeled by iodine had high binding rate with colorectal carcinoma cells.更多还原