【摘要】 针对猪伪狂犬病病毒gE基因保守区域设计并合成了4条引物,建立了伪狂犬病病毒的环介导等温扩增检测方法,并进行了特异性、敏感性和重复性试验。结果显示,该方法可特异性地检测猪伪狂犬病病毒强毒株,不能检测猪繁殖与呼吸综合征病毒、猪细小病毒、猪瘟病毒、猪流感病毒和猪伪狂犬病病毒gE基因缺失疫苗株;该方法的最低检测量可达100个拷贝质粒DNA,比常规PCR方法的敏感性高10倍。试验表明建立的环介导等温扩增方法具有较高的特异性、敏感性和可重复性,可用于伪狂犬病病毒的快速诊断。更多还原

【Abstract】 The objective of this study was to establish a loop-mediated isothermal amplification(LAMP) method for detection of pseudorabies virus(PRV).Four primers were designed according to the PRV gE gene sequence.Then the tests of specificity,sensitivity and reproducibility were carried out.In results,the developed system was specific for the detection of PRV,and there was no cross-reactivity with porcine reproductive and respiratory syndrome virus,porcine parvovirus,classical swine fever virus,swine influenza virus,pseudorabies virus with gE gene deleted.The sensitivity of this assay was 100 copies plasmid DNA and 10 times more sensitive than conventional PCR.These results showed that the LAMP me-thod had higher sensitivity,specificity and reproducibility,and could be used for the detection of PRV.更多还原