【摘要】 选用LMT(Lanzhou mastitis test)检测为阴性的健康乳牛4头8个乳区,通过反相高效液相色谱分析,检测给药后连续7d乳乃康的主要成分阿莫西林在牛奶中残留量的变化,评价药物在体内的残留时间。对样品采用磷酸盐缓冲溶液提取、三氯乙酸沉淀蛋白、固相萃取净化和富集、1,2,4-三唑-氯化汞衍生化。该方法对牛奶中阿莫西林残留的检测限为0.003mg/L。结果显示,休药后第24小时奶样中阿莫西林的平均残留量为12.49μg/L,第48小时的平均残留量为5.92μg/L,第72小时的平均残留量为2.51μg/L,第96小时的平均残留量为0.59μg/L。消除曲线显示,乳乃康的弃奶期宜定为3d。更多还原

【Abstract】 Eight quarters in four healthy cows,which were LMT-negative,were selected to examine dynamic changes of the residual amount of amoxicillin in the cow after Runaikang was administered.Using reversed-phase high performance liquid chromatography(RP-HPLC),the changes of amount of amoxicillin,the main ingredients in the Runaikang,were examined for 7 days in succession after the drug was given and the time of residual in the bodies was evaluated.Samples were extracted by phosphate buffer,precipitated proteins with trichloroacetic acid(TCA) solution,cleaned up with and enriched by solid phase extraction(SPE),and derivatised with 1,2,4-triazole mercury(Ⅱ) reagent.The limit of detection was below 0.03 mg/L.In result,the average amount of residual was 12.49 μg/L at hour 24,5.92 μg/L at hour 48,2.51 μg/L at hour 72 and 0.59 μg/L at hour 96 post-Runaikang-withdrawl.The elimination curve in the experiment indicated that the discard milk period should be 3 days.更多还原