【摘要】 本文旨在研究嗜鞣管囊酵母利用柑橘皮半纤维素水解液发酵生产乙醇的可行性。采用木聚糖酶水解柑橘皮半纤维素,利用嗜鞣管囊酵母(Pachysolen tannophilus)进行木糖发酵生产乙醇;测定嗜鞣管囊酵母细胞生长曲线,乙醇生产及木糖残留曲线,并研究发酵工艺条件。试验结果表明,嗜鞣管囊酵母可有效利用柑橘皮水解液中的木糖进行细胞生长和乙醇生产。以柑橘皮水解液为碳源,发酵生产乙醇是可行的。优化的乙醇发酵生产工艺条件是:发酵时间20h、温度28℃、摇床转速100 r/min、初始pH 4.5、接种量5%。在此工艺条件下,乙醇产量10.1 mg/mL,乙醇得率0.388 g乙醇/g木糖,是理论得率的84.3%。更多还原

【Abstract】 In order to study the feasibility of ethanol produced by fermentation of Pachysolen tannophilus using hemicellulose hydrolyzate of orange peel,the hemicellulose of orange peel were hydrolyzed by xylanase and the xylose of hydrolyzate were fermented by Pachysolen tannophilus.The growth curve of Pachysolen tannophilus and the time course of residual xylose and ethanol produced by Pachysolen tannophilus were determined.The fermentation conditions were also studied.The results showed that Pachysolen tannophilus can ferment xylose in hemicellulose hydrolyzate of orange peel for cells growth and to produce alcohol.It is feasible that hemicellulose hydrolyzate of orange peel used as carbon source to produce ethanol.The optimum technology conditions of fermentation are as follows:fermentation time of 20 h,28 ℃,shaking speed of 100 r/min,pH 4.5,inoculation size of 5%.Under the above conditions,the yield was up to 0.388 g ethanol/g xylose,84.3% of the theoretical yield and the production of ethanol was 10.1 mg/mL.更多还原