【摘要】 目的:探讨阻塞性睡眠呼吸暂停低通气综合症(OSAHS)患者腭咽组织中环氧化酶-2(COX-2)、血管内皮生长因子(VEGF)的表达及其与新生血管生成的关系及意义。方法:经多导睡眠监测仪(PSG)确诊的40例OSAHS患者(其中轻度组7例,中度12例,重度21例)及6例无鼾症患者的软腭组织,采用HE染色光镜观察腭咽部组织的病理组织学改变,免疫组化技术检测COX-2,VEGF及微血管密度(MVD)的表达情况。结果:COX-2、VEGF主要表达于OSAHS患者软腭组织的黏膜鳞状上皮和导管腺上皮,中、重度OSAHS组与对照组比较,COX-2,VEGF,MVD表达均有显著差异(P<0.01),OSAHS组明显高于对照组,轻度组与对照组差别无统计学意义(P>0.05);COX-2、VEGF均与MVD的表达呈正相关(P<0.01),COX-2与VEGF的表达呈正相关(P<0.01),COX-2,VEGF,MVD均与睡眠呼吸暂停低通气指数(AHI)呈正相关(P<0.01),与夜间最低氧饱和度呈负相关(P<0.01)。结论:OSAHS患者腭咽部存在新生血管增生,与缺氧程度有关,COX-2及VEGF在其发生发展过程中可能起到重要作用。更多还原

【Abstract】 Objective:To reseach the correlations between cyclooxygenase-2(COX2) and vascular endothelial growth factor(VEGF) expressions and angiogenesis in pharyngeal tissue of patients with obstructive sleep apnea hypopnea synclnome(OSAHS).Methods:Biopsies were obtained by uvulopalatopharyngoplasty from 40 patients with mild to severe OSAHS.Control specimens of palatopharyngeal and palatoglossal arch mucosa were retreved from 6 patients with chronic tonsillitis and proved have no related disorders.HE was used to observe the changes of pharyngeal tissue,immunohistochemical staining with antibodies against COX-2,VEGF,microvessel density(MVD)(marked with CD34).Results:COX-2 and VEGF mainly expressed at pavement-epithelium and glandular epithelium of pharyngeal tissue,and stronger COX-2 and VEGF expression was found in midrange and severe OSAHS than mild and control group(P<0.01),so as MVD.COX-2 expression was correlated positively with VEGF expression,and had significant correlation with MVD.VEGF expression had the same correlation with MVD.These three targets had considerable relation with apnea hypopnea index(AHI) and lowest O2 saturation at night.Conclusion:There was angiogenesis which had important relationship with hypoxia degree in patients of OSAHS,and COX-2 and VEGF play a crucial role in its development.更多还原