【摘要】 目的:观察吸入适量一氧化碳(CO)对肢体严重缺血/再灌注(I/R)损伤及其所致休克的影响。方法:SD大鼠36只,随机分为I/RI、/R吸入CO(RC)及假手术(S)组。用夹闭双侧股动脉及绑扎后肢根部的办法使后肢缺血8 h,之后行再灌注12 h1、0 d,以此建立肢体严重I/R损伤模型。RC组于再灌注前2 h时开始持续吸入含有CO的医用空气6 h(CO的体积分数为0.075%),S、I/R组呼吸自然空气。观测对比尾动脉压(CAP)、10 d存活率及肢体损伤指标的变化。结果:I/R组再灌注后CAP快速下降,再灌注至8 h时CAP均值降为(5.3259±0.3832)kPa;RC组CAP下降较I/R组速率慢、幅度小,再灌注至8 h时CAP均值为(8.3300±0.4224)kPa。I/R组用于10 d存活率观察的8只大鼠,于再灌注13~20 h间全部死亡,RC组8只中有1只死亡,其余7只再灌注10 d时仍存活。与I/R组比较,RC组肢体的病理学表现明显减轻,肢体湿干重比值、血清乳酸脱氢酶和肌酸激酶活性显著下降(P<0.05)。结论:吸入适量CO对肢体严重I/R损伤所引起的血压下降有反向调节作用,可有效防止休克的发生,减轻肢体损伤,显著提高动物的存活率。更多还原

【Abstract】 Objective:To study the protective effect of carbon monoxide(CO) inhalation on the serious limb ischemia/reperfusion(I/R) injury,and which effects caused to shock in rats.Methods:36 SD rats were randomly divided into I/R,I/R + CO(RC),sham operation(S) groups.I/R injury models were made by the occlusion of the femoral artery for 8 h and the reperfusion for 12 h,10 d.Before reperfusion of 2 h,RC group started to breathe medical air containing CO(the volume fraction of CO:0.075%) continuously,until after reperfusion for 4 h,a total of inhalation 6 h.S,I/R groups exposed to air,breathe freely.Caudal artery pressures(CAP),ten days survival rate,serum lactate dehydrogenase(LDH) and creatine kinase(CK) activity,limb wet-to-dry weight ratio(W/D) and the pathologic changes of limb were observed.Results:Once the reperfusion started, the CAP decreased rapidly in I/R group,and the mean reduced to(5.3259±0.3832)kPa when reperfusion for 8 h.Compared to I/R group,the CAP decreased slower and smaller in RC group,moreover,its mean reduced to(8.3300±0.4224)kPa when reperfusion for 8 h.The 10 d survival rate in I/R group was that 8 rats died all between reperfusion for 13～20 h.Only 1 rat died in RC group and the other 7 rats were still alive when reperfusion for 10 d.Compared to I/R group,the pathological features of the ischemic limb were significant ly improved,and the figures of W/D,serum LDH and CK value were remarkable lower in RC group(P<0.05).Conclusion:Inhaling exogenous low-dose CO has a reverse regulation in the blood pressure decline caused by serious limb I/R injury in rats.And at the same time,it can effectively prevent the occurrence of shock,reduce physical damage,significantly increase the survival rate of animals.更多还原