【摘要】 以16个杂交水稻不育系、恢复系及组合为材料,初步建立了水稻品种SSR荧光标记毛细管电泳检测方法。与常规凝胶电泳检测方法相比,荧光标记毛细管电泳检测方法可以读出目标DNA片段的准确大小,检测数据更为精确,检测效率更高。常规凝胶电泳检测方法验证表明,利用SSR荧光标记毛细管电泳检测方法进行水稻品种DNA指纹鉴定,方法可行、结果可靠。更多还原

【Abstract】 With 16 hybrid rice male sterile lines,restorer lines and combinations as materials,a capillary electrophoresis detection method with fluorescent SSR markers in rice was primarily developed.The accurate size of DNA fragment could be detected by the capillary electrophoresis detection method with fluorescent SSR markers.The capillary electrophoresis detection has the advantages of data accuracy and efficiency compared with polyacrylamide gel electrophoresis detection.It provides a feasible and reliable method for rice DNA fingerprint identification.更多还原