【摘要】 利用已公布的OsCRY1a基因的序列设计引物,扩增部分基因片段,构建RNAi植物表达载体并转化水稻,导致该基因表达水平下降和功能缺失。根据转基因植株重要农艺性状的表现,分析该基因的功能。结果表明,抑制OsCRY1a基因的表达,水稻开花期推迟16d,株高和粒长明显增加,而其他重要农艺性状如剑叶长、宽和穗长等无明显变化。更多还原

【Abstract】 Using primers designed according to the published sequence of OsCRY1a gene,part of the gene fragment was obtained by PCR and a RNAi expression vector was constructed.The vector was then introduced into rice,leading to the downregulation of the expression level and loss-of-function of the gene.Based on the performance of the transgenic plants,the functions of OsCRY1a was analyzed and deduced.The results indicated that suppressing the expression of OsCRY1a retarded flowering for 16 d with the plant height and grain length significantly increased while other important agronomic traits remained unchanged apparently.更多还原