【摘要】 采用蛋白质组学分析技术,对养分胁迫下杂交水稻威优916功能叶片蛋白质组差异表达进行研究,以揭示养分胁迫影响籽粒灌浆期水稻衰老的分子机理。经双向电泳分离,从功能叶中获得34个差异表达蛋白质,其中26个蛋白质功能得到鉴定,包括11个参与光合作用蛋白质,4个参与呼吸作用蛋白质,10个参与抗氧化及信号传导蛋白质和1个未知功能蛋白质。分析结果表明,养分胁迫下,灌浆期水稻叶片光合作用及呼吸作用显著下降;清除活性氧类蛋白质表达量下调,活性氧生成蛋白质及胁迫信号传导蛋白质表达量上调,水稻抗性减弱,叶片中活性氧积累,水稻衰老加剧。更多还原

【Abstract】 In order to reveal the molecular senescence mechanism of rice under nutrient stress at grain filling stage,the protein expression profile of functional leaves in hybrid rice Weiyou 916 was investigated by the approach of proteomics.Proteins were extracted from rice flag leaf and separated by two-dimensional gel electrophoresis.Thirty-four differentially expressed proteins were obtained,among which 26 were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry,and categorized into four groups of functions: 11 proteins were associated with photosynthesis,four proteins were associated with respiration,10 proteins were related to antioxidative pathways and signal transmitting and one protein was putative protein.The functions of these 26 proteins were analyzed and the conclusion could be drawn from the following results: under nutrient stress conditions,photosynthesis and respiration of rice leaf declined significantly;proteins associated with antioxidative pathways decreased in expression abundance,and proteins associated with active oxygen synthesis and signaling showed drastical increase in expression abundance,which resulted in reduced resistance and accumulated active oxygen,consequently accelerated rice senescence process.更多还原