【摘要】 目的探讨不同病理分级、临床分期的子宫内膜癌中肿瘤浸润性树突状细胞(TIDC)临床表达及其临床意义。方法 2005年1月至2009年10月在暨南大学附属第一医院对45例子宫内膜癌组织中TIDC行S-100单克隆抗体免疫组织化学染色,通过体视学参数分析TIDC在子宫内膜癌中的浸润状况;采用流式细胞仪检测TIDC膜表面MHCⅡ-分子及CD54的表达。结果子宫内膜癌中TIDC数量显著少于正常子宫内膜组织中树状突细胞(DC)数量(数密度参数比为:9.81±1.03 vs 19.37±1.14,P<0.05);不同病理分级子宫内膜癌中TIDC数量有统计学意义(即高分化、中分化、低分化子宫内膜腺癌TIDC数密度参数比:11.67±1.98 vs 7.08±1.17 vs 3.36±0.28,P<0.05);不同临床分期子宫内膜癌中TIDC数量有统计学意义(Ⅰ期、Ⅱ期、Ⅲ~Ⅳ期子宫内膜癌中TIDC数密度参数比:12.85±2.01 vs 6.89±1.07 vs 3.85±0.56,P<0.05);子宫内膜癌组织TIDC膜表面MHCⅡ-分子、CD54的表达显著低于正常子宫内膜组织[(5.92±1.14)%vs(11.09±1.26)%,(6.08±1.18)%vs(10.87±1.23)%,P<0.05]。结论随着病理分级、临床分期的增加,TIDC浸润数目减少、抗原提呈功能降低或缺失可能导致子宫内膜癌免疫逃逸发生。TIDC可作为子宫内膜癌患者判断预后的指标之一,为其免疫治疗提供依据。更多还原

【Abstract】 Objective To investigate the expression of tumor infiltrating dendritic cells(TIDC) and the relationship between histologic grade,clinical stage and TIDC invasion in human endometrioid adenocarcinoma.Methods Compared with 20 cases of human normal endometrium tissues in the postmenopause in the first Affiliated Hospital of Jinan University between January 2005 and October 2009,the dendritic cells were measured in 45 cases of endometrioid adenocarcinoma by immunohistochemistry using the monoclonal antibody to S-100 protein.The expressions of HLA-DR and CD54 were analyzed by flow cytometry.Results In comparison with the control group,the volume density of DC in human endometrioid adenocarcinoma were significantly lower(9.81±1.03vs19.37±1.14,P<0.05).In human endometrioid adenocarcinoma,there was significant decrease in the volume density of TIDC in well-differentiated group,in mid-differentiated group and poorly-differentiated group(11.67±1.98vs7.08±1.17vs3.36±0.28,respectively,P<0.05).The same result was found in clinical stageⅠ,clinical stageⅡ and clinical stageⅢ～Ⅳ(12.85±2.01vs6.89±1.07vs3.85±0.56,respectively,P<0.05).The expression levels of MHC-Ⅱand CD54 of DC were considerablely lower than in human endormetrioid adenocarcinoma than that in normal endometrium[(5.92±1.14)%vs(11.09 ±1.26)%,(6.08±1.18)%vs(10.87±1.23)%,respectively,P<0.05].Conclusion With the histologic grades or clinical stages rising,the decrease of the amounts of dysfunctional TIDC in human endometrioid adenocarcinoma may lead to tumor immune escape.TIDC could be considered as a marker to evaluate the prognosis of human endometrioid adenocarcinoma.更多还原