【摘要】 模拟工厂化生产豆芽工艺培育黄豆芽,对培育过程中出现的烂芽病样进行病原菌的分离、纯化及鉴定,确定病原菌为立枯丝核菌(Rhizoctoniasolani);并测定了立枯丝核菌不同菌量对绿豆芽的致病性,结果显示:不同接种量引起绿豆芽的发病时间、发病率及生物产量均存在明显差异。接种量为2块菌饼配制5mL和2块菌饼配制10mL的菌丝悬浮液造成严重的烂芽,第5天发病率分别达46%和25%。不同处理防治豆芽立枯病的试验表明:40℃热力消毒、1%石灰水和1/5000高锰酸钾均有一定的防治作用,相对防治效果分别为91.30%、54.35%和50.00%。更多还原

【Abstract】 Simulating factory production process of sproutsing to cultivate bean sprouts,taking spoilage sprouts samples during the process of cultivating,then separating,purifying and identification were carried out to determine the pathogen.Finalized for the pathogen Rhizoctonia solani was finalized.Observing the pathogenicity of inoculation volume of different Rhizoctonia mycelial suspensions on bean sprouts was tested.The results showed that different inoculums leaded to the differencest of disease occurring the time of incidence,the incidence rate and bean sprouts biological production.Using inoculums at 2 pieces bacteria per 5 milliliter and 2 pieces bacteria per 10 milliliter of mycelial suspensions to treat bean sprouts,the spoilage of bean sprouts was serious.The incidence rate at the 5th day were 46 %,25 %,respectively.The results of using different treatments to protect Rhizoctonia solani showed that 40 ℃ hot-water disinfection,1 % lime water,1/5 000 potassium permanganate might control the occurrence of Rhizoctonia to some extent,the relative control efficiencies were 91.30 %,54.35 %,50.00 %,respectively.更多还原