【摘要】 目的:探讨超声波治疗对兔膝骨关节炎(KOA)模型软骨细胞基质金属蛋白酶-1(MMP-1)表达的影响。方法:30只新西兰大白兔随机分为正常对照组、模型组和超声波治疗组,每组10只。将模型组和超声波治疗组的兔左膝于伸直位石膏固定6周制作KOA模型。造模后超声波治疗组实施超声波治疗2周。8周后取各组软骨观察膝软骨病理学形态改变、MMP-1表达的变化。结果:采用Mankin评分比较病理学形态改变,正常对照组与模型组、模型组与超声波治疗组间比较,差异均有显著性意义(P<0.01)。软骨免疫组化MMP-1测定,正常对照组与模型组、模型组与超声波治疗组间比较,差异均有显著性意义(P<0.01)。结论:超声波能有效治疗膝骨性关节炎,其作用机制可能与减少软骨细胞MMP-1的含量而利于软骨细胞的修复有关。更多还原

【Abstract】 Objective:To study the effect of ultrasound therapy on expression of matrix-metalloproteinase-1(MMP-1) in cartilage cells of rabbit with knee osteoarthritis(KOA).Method:Thirty healthy New Zealand white rabbits were randomly divided into 3 groups:normal control group contained 10 rabbits,not modeling;the other 20 rabbits were randomly divided into model group and ultrasonic experimental group,being created osteoarthritis.The rabbits’ left knees were fixed within plaster cast in stretch position for 6 weeks to created KOA models.Then ultrasonic,experimental group was given ultrasonic treatment for 2 weeks.After 8 weeks cartilage changes on pathological morphology and expression of MMP-1 were observed in all groups.Result:By Mankin’s score the severity of pathological lesion in model group was higher than that in normal control group and ultrasonic experimental group(P<0.01).The expression of MMP-1 in model group was higher than that in normal control group and ultrasonic experimental group,the difference was significant(P<0.01).Conclusion:The ultrasonic treatment can effectively improve the rabbits’ KOA,and its mechanism probably is correlated with reducing MMP-1 level in cartilage cells,which will be helpful for repairing cartilage cells.更多还原