【摘要】 目的了解福建省流行的麻疹野病毒基因型别及特征。方法应用B95a细胞[Epstein-Barr(EB)Virus-Transformed,Marmoset B Lymphoblastoid Cell Line,埃泼斯坦-巴尔病毒转化的绒猴淋巴母细胞]或Vero/SLAM细胞[Vero Cells Transfected to Express the Human Signaling Lymphocyte Activation Molecule(SLAM),淋巴信号激活因子转染的非洲绿猴肾细胞],从疑似麻疹病例的咽拭子或尿液标本分离麻疹病毒,用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)扩增分离株病毒的核蛋白(Nucleoprotein,N)基因3’端450个核苷酸片段,分析其基因型别及遗传特征。结果 2002年、2006～2009年,共从6个设区的市分离了24株麻疹野病毒,均为H1基因型、H1a基因亚型,未分离到其他型别的毒株。福建株间核苷酸同源性为97.8%～100.0%,在遗传树图中属于4个独立分支谱系(Lineage),Lineage4分支病毒出现了3个新的氨基酸突变位点。福建株中来自不同地区和年份的MV(Measles Virus,麻疹病毒)07-29FZ(Fuzhou,福州)、MV07-20ND(Ningde,宁德)、MV07-47ND和MV08-78QZ(Quanzhou,泉州)、MV06-32LY(Longyan,龙岩)、MV07-30FZ、MV07-39FZ和MV07-46ND的N基因3’端的450核苷酸同源性均为100.0%。福建株与H1基因型中国代表株、H2基因型及疫苗株S(Shanghai,上海)191相比,与S191疫苗株的A基因组差异最大(核苷酸同源性90.1%～93.3%,氨基酸同源性86.8%～91.4%)。结论 H1a为福建省本土麻疹野病毒优势基因亚型,未发现其他基因亚型。近3年的分离株出现了新的氨基酸位点突变。不同地区或同一地区内存在多传播链同时循环,也存在同一野病毒株在不同地区、不同年份持续循环。更多还原

【Abstract】 Objective-To analyse of the characteristic of the indigenous measles virus(MV)in Fujian before 2012.Methods-MV were isolated from the throat swabs or urine specimens of measles patients by the B95a or Vero/SLAM cell line and identified by RT-PCR.The 450 nucleotide sequence of C terminus of the nucleoprotein(N)gene was amplified and directly sequenced.Phylogenetic analysis were performed.Results-24 strains were isolated from Fuzhou,Ningde,Quanzhou,Longyan and Sangming cities during 2002,2006～2009.All the isolates were all identified as H1 genotype,H1a subgenotype,which belong to 4 different Lineages in the phylogenetic tree.Comparing with the other strains in the H1a,the strains of Lineage 4 got 3 new mutation sites of Amino Acids.The sequence identity matrix of the Fujian isolates showed that the homology of nucleotide sequence was 97.8%～100%.Fujian strains of MV07-29FZ(Fuzhou,FZ)、MV07-20ND(Ningde,ND)、MV07-47ND and MV08-78QZ(Quangzhou,QZ),MV06-32LY(Longyan,LY)、MV07-30FZ、MV07-39FZ and MV07-46ND,which isolated from different years and prefectures,had the same 3’ terminal sequence of 450 nucleotide respectively.The homology of nucleotide and amino acid sequences were 90.1%～93.3% and 86.8%～91.4% respectively compared with genotype A(including S191).Conclusions-H1a sub-genotype was the pre-dominant genotype of wild measles virus circulated in Fujian Province during 2002,2006～2009,other sub-genotypes were not found.Strains isolated in recent 3 years got new mutation sites of amino acids.There were several different transmission chains caused measles co-circulations in the same or different prefectures in Fujian province,and there was one wild MV which could induce outbreaks or epidemiology in different cities for several years.更多还原