【摘要】 目的探讨维生素A缺乏对大鼠铁调节蛋白2(IRP2)mRNA及铁蛋白(Fn)mRNA和转铁蛋白受体(TfR)mRNA表达影响。方法 48只雄性SD大鼠按体重随机分为4组,每组12只,对照组、维生素A完全缺乏组;维生素A边缘缺乏组;铁及维生素A边缘缺乏组;喂养8周后,麻醉处死大鼠,取组织和血清,进行相关指标和基因表达检测。结果与对照组(1.50±0.41)μmol/L比较,维生素A完全缺乏大鼠血清维生素A(0.22±0.26)μmol/L水平明显降低(P<0.05);与对照组比较,维生素A完全缺乏组大鼠血清铁(2.26±0.72)μg/mL明显降低;维生素A完全缺乏组大鼠肝脏IRP2、TfR表达水平分别为(1.53±0.18)、(0.62±0.06)与对照组相比明显升高,FnmRNA(0.52±0.08),明显低于对照组(P<0.05)。结论维生素A缺乏通过改变IRP2表达、IRP-RNA结合活性,在转录后水平改变TfR和Fn mRNA表达,影响铁稳态。更多还原

【Abstract】 Objective To explore the potential role of iron regulator protein 2(IRP2) under conditions of vitamin A deficiency and the relationship between vitamin A deficiency and anemia.Methods Forty-eight SD rats were divided randomly into four groups of control,complete deficient in vitamin A,marginal deficiency in vitamin A,and ion plus vitamin A marginal deficiency.After 8 weeks,all the rats were killed by sodium pentobarbital anesthesia and all samples were collected and detected for gene expression.Results As expected,the concentration of serum retinol in completely vitamin A deficiency group(0.22±0.26 μmol/L),compared with that of the control group(1.50±0.41μmol/L),was significantly decreased.Compared with that of the control group(3.85±0.94μg/ml),the concentration of serum rion(2.26±0.72 μg/ml) in complete vitaminA deficiency group was significantly decreased.Compared with that of the control group(0.81±0.08),the level of IRP2 mRNA(1.53±0.18)in complete vitamin A deficiency group was significantly increased,and the level of transferrin receptor mRNA(0.62±0.06) was also increased compared with that of the control group(0.33±0.02).Compared with the control group(0.85±0.04),the level of ferrintin RNA(0.52±0.08) in complete vitamin A deficiency group was significantly decreased.Conclusion The results indicate that vitamin A deficiency influences iron homeostasis in cells through affecting the expression of IRP2 and the activity of IRP-RNA comhinatiion and then changing the expressions of ferritin and transferrin mRNA.更多还原