【摘要】 目的研发高效的锰中毒解毒剂。方法小鼠一次性腹腔注射MnCl2(100 mg/kg),24 h后分别给予L-组氨酸(L-His)、乙二胺四乙酸(EDTA)、二乙基二硫代氨基甲酸钠(DDTC)、meso-2,3-二巯基丁二酸(DMSA)及N-乙酰-L-半胱氨酸(NAC)等五种螯合剂(2 mmol/kg,腹腔注射),观察小鼠血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)和乳酸脱氢酶(LDH)活性、肝脏脂质过氧化物值(LPO)和肝脏组织中锰、钙、锌浓度变化。结果染锰2 d后小鼠血清AST、ALT和LDH活性分别达(251.00±74.28)、(141.00±21.04)、(1393.51±202.01)U/L,肝脏中LPO值为(2.88±0.29)μmol/g prot,锰浓度(18.34±4.74)μg/g,明显高于对照组,差异有统计学意义(P<0.05),L-His和NAC对染锰引起的血清LDH、AST、ALT活性及肝LPO和锰含量升高有明显抑制作用。结论 L-His和NAC对锰致小鼠肝脏毒性有较好解毒作用,有望成为理想的锰解毒剂。更多还原

【Abstract】 Objective To develop a new antidotes for manganese(Mn).Methods Mice were injected intraperitoneally with MnCl2(100mg MnCl2/kg).Twenty-four hours after the MnCl2 treatment,the mice were injected intraperitoneally with one of the five chelating agents(2 mmol/kg for each),such as L-histidine(L-His),ethylene diaminetetraacetic acid(EDTA),diethyldithiocarbamate(DDTC),meso-2,3-dimercapto succinc acid(DMSA),and N-acetylcysteine(NAC),respectively.The effects of the chelating agents on activities of lacatate dehydrogenase(LDH),aspartate transcarbamoylase(AST) and alanine aminotranferase(ALT) in serum,lipid peroxidation(LPO),and concentrations of Mn,Ca,Zn in liver of the mice induced by the acute manganese exposure were studied.Results Mn injection significantly increased the activities of AST(251.00±74.28),ALT(141.00±21.04),and LDH(1393.51±202.01IU/L) in serum,and LPO level(2.88±0.29 μmol/g prot),as well as the concentrations of Mn(18.34±4.74 g/g wet tissue) in the liver of the mice(P<0.05) two days after the MnCl2 injection.L-His and NAC effectively prevented the increases of the indicators.Conclusion L-His and NAC are effective detoxification for the liver toxicity induced by acute Mn exposure in the mice and could be used as antidotes of manganismus.更多还原