【摘要】 2008年从临床鸽病例中分离到2株新城疫病毒毒株,经测定,鸡胚平均死亡时间(MDT)分别为48h和46h,8周龄SPF鸡静脉接种指数(IVPI)分别为2.88和2.91,一日龄雏鸡脑内接种指数(IPCI)分别为1.88和1.89,按照OIE推荐的标准均为强毒株。RT-PCR扩增出融合蛋白(F)基因片段,测序,推导的氨基酸序列中蛋白酶裂解位点附近的氨基酸序列均符合强毒特征序列。根据F蛋白的部分基因序列绘制的系统进化发生树和F基因片段中3种限制性内切酶(RE)位点分布判定这2个毒株均为基因Ⅶc型。更多还原

【Abstract】 Two Newcastle disease virus isolates were got from dead pigeons in 2008.The virulence of the isolates were identified based on the MDT,ICPI and IVPI.The genes of fusion protein were sequenced.The amino acid sequence between 112 to 117 were 112R(K)RQKR↓ F117.The restriction enzyme cleavage site mapping of the fusion protein gene between nucleotides 334 and 1682 and by sequence analysis between nucleotides 47 and 420.Both methods revealed the two isolates belong to genotype Ⅶc.The characters of the isolates were as same as other isolates of this genotype from geese and chickens in recent years.It was shown that the virulence and genotype of the Newcastle disease virus from pigeons had changed.更多还原