【摘要】 停乳链球菌是引起奶牛乳房炎的主要病原菌,根据GenBank中登陆的停乳链球菌表面蛋白MIG基因序列,设计一对引物,采用PCR的方法从临床分离的停乳链球菌内蒙分离株基因组DNA中扩增出MIG基因,得到一条1900bp的片段。将其连入PMD-19T载体中,经酶切,PCR及序列测定法进行鉴定。经DNA测序分析,证实与GenBank中停乳链球菌MIG基因(AF354651)序列同源性为95%,具有高度保守性。更多还原

【Abstract】 S.dysgalactiae is the major pathogens of mastitis in dairy cows.S.dysgalactiae were derived from the mastitis bovine in some areas of Inner Mongolia.A pair of primers was designed according to S.dysgalactiae MIG surface protein gene sequences in GenBank.Subsequently,The MIG gene of 1900 bp was amplified by PCR.Then the gene was cloned into PMD-19T vector,identified by digestion with restriction endonucleases,PCR and sequencing.The DNA sequencing confirmed S.dysgalactiae in GenBank,MIG gene(AF354651) 95% homology.更多还原