【摘要】 运用同源基因克隆技术从典型的克隆植物赖草中克隆了其根茎分化相关基因LRC1,通过RACE获得其全长cDNA为1.598kb,开放阅读框(ORF)为1299bp,编码432个氨基酸。同源分析结果显示,赖草LRC1基因属于GRAS转录因子家族,与控制水稻分蘖及烟草侧枝发生的相应蛋白具有很高的同源性,与控制水稻分蘖的MOC1基因同源性达到91%。实时定量检测结果表明,LRC1基因在赖草初花期的根茎节、根茎节间、根茎尖和穗、茎、叶片6个组织部位均有表达,但在根茎中的表达量显著高于其它部位,特别是在分蘖产生的根茎节其表达量是叶片的43倍。更多还原

【Abstract】 A new tillering gene(named LRC1) was cloned from Leymus secalinus through homologous cloning.The full length of cDNA of gene LRC1 was 1598bp,and was highly homologous to MOC1 gene(91% similarity) which was considered to be the tillering gene in rice.The open reading frame of LRC1 was 1299bp,coding 432 amino acids.Homologous analysis revealed that the protein belonged to GRAS family,with a high homology to transcription factor such as MOC1(controlling tillering) and LS(controlling axilare meristem) in rice and tobacco respectively.The expression examination of LRC1 with real time PCR showed that the gene was expressed in the node of rhizome,internode of rhizome,top of rhizome,ear,stem and leaf of Leymus secalinus at the stage of initial bloom.However,the expression levels of LRC1 were quite different,the highest expression level was found in rhizome(the expression level in the node of rhizome was about 43 times than in leaves).The results paved the way to elucidate the mechanism of gene regulation for tillering in cloning plants and how to control tillering of rhizome plants artificially.更多还原