【摘要】 目的:了解婴幼儿人巨细胞病毒(HCMV)活动性感染的临床表现,以及与糖蛋白B(gB)基因型的关系。方法:ELISA法检测HCMV-IgM确定的HCMV阳性的婴幼儿51例,对其不同临床症状进行分析。对其中43例患儿使用套式PCR(nPCR)法加限制性长度多态性分析(RFLP)进行HCMV gB基因分型。结果:在51例HCMV感染患儿中全身性感染和单脏器感染分别占25.49%和74.51%,HCMV包涵体病和肝炎分别占25.49%和21.57%。43例患儿HCMV gB的基因分型结果为,gBI型20例,gB II型7例,gB III型9例,gB I、II混合型4例,gB I、III混合型2例,gB II、III混合型1例,未发现gB IV型。gB I型占46.51%。结论:婴幼儿HCMV感染临床表现种类多样;HCMV感染者以gB I型HCMV占多数。更多还原

【Abstract】 Objective:To understand the clinical manifestations of human cytomegalovirus(HCMV) activate infection of infants and its relationship with genotype of glycoprotein B.Methods:ELISA method was used to detect 51 infants with positive HCMV diagnosed by HCMV-IgM,and the different clinical symptoms were analyzed.Genotyping of HCMV glycoprotein B was performed among 43 infants by nested PCR and restriction fragment length polymorphism(RFLP).Results:Among 51 infants with HCMV infection,25.49% of them were systemic infection and 74.51% of them were single organ infection,the proportions of HCMV inclusion disease and hepatitis were 25.49% and 21.57%,respectively.The results of genotyping of HCMV glycoprotein B among 43 infants:20 infants with glycoprotein B I genotype,7 infants with glycoprotein B Ⅱ genotype,9 infants with glycoprotein B Ⅲ genotype,4 infants with glycoprotein B I genotype and glycoprotein B Ⅱ genotype,2 infants with glycoprotein B I genotype and glycoprotein B Ⅲ genotype,one infant with glycoprotein B Ⅱ genotype and glycoprotein B Ⅲ genotype,no infant was found with glycoprotein B IV genotype;glycoprotein B I genotype accounted for 46.51%.Conclusion:The clinical manifestations of infantile HCMV infection are various;glycoprotein B I genotype is in the majority among HCMV infected infants.更多还原