【摘要】 目的研究依达拉奉对脂多糖(LPS)诱导的帕金森病(PD)大鼠模型的保护作用及机制。方法大鼠黑质内立体定向注射14μg LPS建立PD大鼠模型。选取造模成功PD大鼠24只,随机分成大、小剂量组和未治疗组。3组大鼠分别接受不同剂量的依达拉奉注射液(5.0mg/kg,1.0mg/kg)或生理盐水;另外选取正常大鼠5只,作为正常对照组。注射4w后观察各组大鼠黑质酪氨酸羟化酶(TH)阳性细胞的数量以及超氧化物歧化酶(SOD)及丙二醛(MDA)表达变化。结果与未治疗组相比,依达拉奉大、小剂量组TH阳性细胞数显著增多,SOD表达也明显增多(P<0.05),MDA表达明显减少(P<0.05)。结论依达拉奉可能通过减少LPS诱导的自由基产生而对PD大鼠发挥神经保护作用。更多还原

【Abstract】 Objective To explore the protective effect of the edaravone on dopaminergic neurons of the PD rats induced by lipopolysaccharide(LPS).Methods The rat model of PD was established after intranigral injection of 15μg LPS.Twenty-four female PD rats were randomly divided into three groups with different injection dose of edaravone(5.0mg/kg,1.0mg/kg)and the injection of saline as control.Five normal rats were set as normal group.On 28d,the number of tyrosine hydroxylase(TH)positive neurons and superoxide dismutase(SOD)and malondialdehyde(MDA)in the substantia nigra(SN)were observed.Results The TH positive neurons survived in the animals treated with edaravone were more than those of the saline group(P<0.05).The SOD levers of edaravone groups was higher,while MDA level was lower than the saline group(P<0.05).Conclusion Edaravone attenuates LPS-induced degeneration of rat substantia nigral dopaminergic neurons.更多还原