【摘要】 目的研究茶多酚对大鼠肠缺血再灌注(I/R)后血浆和肺细胞间黏附分子-1(ICAM-1)蛋白表达的影响。方法 SD雄性大鼠32只随机分4组(n=8):①I/R组:夹闭肠系膜上动脉(SMA)1h后开放2h;②预处理组(P1):缺血前10min予茶多酚;③处理组(P2):再灌注前10min予茶多酚;④假手术组:仅暴露SMA。茶多酚首剂10mg/kg,后以10mg/(kg.h)持续输注。用酶联免疫吸附法和免疫组化法测定。结果肠I/R后血浆ICAM-1含量增加,肺组织ICAM-1蛋白表达明显增加。而茶多酚可以抑制上述改变,以P1组效果最为明显。结论 ICAM-1在肠I/R后肺损伤的发生中发挥重要作用。肠I/R早期应用茶多酚可更明显减少肺组织ICAM-1表达,在一定程度上减轻肺损伤。更多还原

【Abstract】 Objective To investigate the effect of tea polyphenols on intercellular adhesion molecular-1(ICAM-1)expression in plasma and the lung tissue following intestinal ischemia/reperfusion(I/R)in rats.Methods 32 SD rats were randomly divided into 4 groups(n=8).①Group I/R in which rats were subjected to 1 hour of occlusion of the superior mesenteric artery(SMA),followed by 2 hours of reperfusion.②Early treatment group(P1),rats were subjected to the same procedure as group I/R with the additional administration of tea polyphenols beginning 10 minutes before ischemia with 10mg/kg loading dose,followed by continuous infusion at 10mg/(kg.h).③Treatment group(P2),rats were subjected identical insult as in group I/R with the administration of tea polyphenols started 10minutes before reperfusion with 10mg/kg loading dose,followed by continuous infusion at 10mg/(kg·h).④ Sham-operation group,rats were subjected to only.The ICAM-1 in plasma was assessed by ELISA.The ICAM-1 expression in pulmonary endothelium was assessed by histochemical staining.Results ICAM-1 in plasma and expression in lung tissue was increased significantly in group I/R and was decreased significantly by tea polyphenols.All the increase was less in quantity in the early treatment group of tea polyphenols than the other treatment group.Conclusion ICAM-1 plays an important role in lung injury after intestinal I/R.The early treatment of tea polyphenols before intestinal I/R may be more beneficial by reducing ICAM-1 expression in lung injury.更多还原