【摘要】 目的应用Box-Behnken实验设计,优化水飞蓟素固体脂质纳米粒的最佳处方。方法采用三因素三水平Box-Behnken实验设计,以水飞蓟素为模型药物,采用乳化蒸发-低温固化法制备固体脂质纳米粒。利用效应曲面法对影响固体脂质纳米粒包封率、载药量和粒径的主要因素进行考察,以包封率、载药量和粒径为响应值,建立相应的二项式数学模型优化处方。结果最优处方为固体脂质纳米粒中脂质单硬脂酸甘油酯量为5.05%,7.25%Poloxmer 188作为乳化剂,药物的量为15%。结论采用Box-Behnken实验设计可用于水飞蓟素固体脂质纳米粒的处方优化筛选。更多还原

【Abstract】 Objective To optimize the formulation parameters of solid lipid nanoparticles(SLN) of silymarin by Box-Behnken experimental design.Methods A three-factor and three-level Box-Behnken experimental design was employed using emulsion evaporation-low temperature solidification technique to prepare SLN with silymarin as model drug.Response surface methodology was used to investigate the factors affecting entrapment efficiency(EE),drug loading(DL),and particle size.Binomial mathematical model-optimized formulation was established with EE,DL,and particle size as response values.Results The optimal formulation was as follows: the amount of glycerol monostearate was 5.05%,the concentration of Poloxamer 188 was 7.25%,and the amount of drug was 15%.Conclusion The Box-Behnken experimental design could be used to optimize the SLN of silymarin.更多还原