【摘要】 目的测定穿龙骨刺片(穿山龙,淫羊藿,狗脊,川牛膝,熟地黄,枸杞子)中薯蓣皂苷、淫羊藿苷。方法采用高效液相色谱法,色谱柱为C18柱,以乙腈-水为流动相,检测波长为203 nm(薯蓣皂苷)、270 nm(淫羊藿苷)。结果薯蓣皂苷进样量、淫羊藿苷进样量分别在0.56～5.60μg0、.24～2.40μg范围内与其峰面积积分值呈良好线性关系(r分别为0.999 9、0.999 8);平均回收率分别为98.23%、98.34%,RSD分别为0.88%(n=6)、0.62%(n=6)。结论 HPLC法快速、准确,可用于该制剂的质量控制。更多还原

【Abstract】 AIM To determine the contents of dioscin and icariin in Chuanlong Guci Tablet(Dioscoreae nipponicae Rhizoma,Epimedii Folium,Cibotii Rhizoma,Cyathulae Radix,Rehmanniae Radix Praeparata,Lycii Fructus).METHODS HPLC method was adopted and a C18 column was used.The mobile phase was acetonitrile-water.The detection wavelengths were set at 203 nm(dioscin)、and 270 nm(icariin).RESULTS The linear ranges were 0.56-5.60 μg(r=0.999 9) for dioscin,0.24-2.40 μg(r=0.999 8) for icariin,respectively.Their average recoveries were 98.23%(RSD was 0.88%),98.34%(RSD was 0.62%).CONCLUSION The method is quick and accurate,and it can be used for quality control of Chuanlong Guci Tablet.更多还原