【摘要】 目的:检测1,25-二羟维生素D3[1,25-(OH)2D3]对被动致敏的人气道平滑肌细胞(HASMCs)增殖的调节作用,探讨其对哮喘气道重塑的可能作用。方法:离体培养HASMCs,并将细胞分为对照组、哮喘组及1,25-(OH)2D3组。MTT法检测细胞增殖活力并确定1,25-(OH)2D3最佳作用浓度;用最佳作用浓度刺激HASMCs 48 h后以流式细胞术测定细胞周期,免疫细胞化学染色(SABC法)检测增殖细胞核抗原(PCNA)的表达。结果:1,25-(OH)2D3在10-9-10-7 mol/L范围内能显著抑制哮喘血清被动致敏的HASMCs增殖(P<0.05),且10-7 mol/L时抑制作用最强;流式细胞术检测显示这一最佳作用浓度的1,25-(OH)2D3能显著抑制被动致敏HASMCs由G0/G1期向S期的转化;此外,1,25-(OH)2D3能显著抑制被动致敏HASMCs中PCNA的表达。结论:1,25-(OH)2D3能抑制哮喘血清被动致敏的HASMCs增殖,有助于哮喘气道重塑的防治。更多还原

【Abstract】 AIM: To investigate the effects of 1,25-dihydroxyvitamin D3[1,25-(OH)2D3] on the proliferation of passively-sensitized human airway smooth muscle cells(HASMCs),and to explore its potential role in asthmatic airway remodeling.METHODS: HASMCs were passively sensitized with 10% serum from asthmatic patients.1,25-(OH)2D3 was used as the interventor.The effect of 1,25-(OH)2D3 on the cell proliferation and its optimal concentration were determined by MTT colorimetric assay.The cell cycle analysis was performed by flow cytometry.The expression of proliferating cell nuclear antigen(PCNA) was measured by the method of immunocytochemical staining.RESULTS: 1,25-(OH)2D3 at the concentrations of 10-9-10-7 mol/L markedly inhibited the cell proliferation and the maximum effect was observed at the concentration of 10-7 mol/L.This concentration of 1,25-(OH)2D3 markedly suppressed the PCNA-positive rate and hampered the G1/S transition in HASMCs passively-sensitized by asthmatic serum.CONCLUSION: 1,25-(OH)2D3 has direct inhibitory effects on the proliferation of passively-sensitized HASMCs in vitro,which may be concerned with the beneficial role of 1,25-(OH)2D3 on the prevention and therapy of asthmatic airway remodeling.更多还原