【摘要】 目的:了解职业苯接触及慢性苯中毒工人外周血中T细胞特异性转录因子T-bet和GATA-3mRNA表达情况。方法:利用SYBR Green I实时荧光定量PCR分别检测20例正常人、25例职业苯接触工人和27例慢性苯中毒工人外周血单个核细胞T-bet和GATA-3 mRNA表达情况。结果:在职业接触苯工人组及慢性苯中毒工人组,多数样本表现为GATA-3表达上升和T-bet表达下降,而少数病人则表现为相反的模式。GATA-3在正常人的表达水平为0.39±0.22,与正常组对照比较职业接触苯工人组中有19例GATA-3表达水平呈上升趋势(0.57±0.54),慢性苯中毒工人组中则有20例GATA-3表达水平呈上升趋势(0.52±0.50)。此外,在职业接触苯工人组中发现6例GATA-3表达水平显著下降(0.15±0.12,P<0.05),同样在慢性苯中毒工人组中也有7例GATA-3表达水平显著下降(0.07±0.06,P<0.05)。T-bet在正常人的表达水平为2.15±1.45,与正常组对照比较职业接触苯工人组中有19例T-bet表达水平呈下降趋势(1.91±1.49),慢性苯中毒工人组中T-bet表达水平均呈下降趋势(1.52±0.56)。此外,在职业接触苯工人组中也可发现6例T-bet表达水平显著上升(3.19±2.10,P<0.05)。结论:职业苯接触及慢性苯中毒影响工人外周血中T细胞特异性转录因子T-bet和GATA-3mRNA表达水平。更多还原

【Abstract】 AIM: To investigate the mRNA expression level of T cell-specific transcription factors T-bet and GATA-3 in peripheral blood of benzene-exposed and poisoning workers. METHODS: The mRNA expression of T-bet and GATA-3 in peripheral blood mononuclear cells(PBMCs) from 20 normal individuals,25 benzene-exposed workers and 27 benzene poisoning workers was quantitatively detected by real-time PCR using SYBR green I.RESULTS: Mostly,the mRNA expression of GATA-3 showed an increased trend while T-bet showed a decrease in PBMCs in benzene-exposed group and poisoning group.However,a few cases still showed an opposite pattern.The mRNA expression of GATA-3 in normal group was 0.39±0.22,in 19 cases of benzene exposed workers was 0.57±0.54,and in 20 cases of benzene poisoning workers was 0.52±0.50.GATA-3 expression levels of benzene-exposed workers(6 cases) and of poisoning workers(7 cases) were 0.15±0.12 and 0.07±0.06,respectively,significantly lower than that in normal group(P<0.05).T-bet expression levels were 1.91±1.49 in benzene-exposed group(19 cases) and 3.19±2.10 in benzene-exposed group(6 cases).The latter was significantly higher than that in normal group(2.15±1.45,P<0.05).The expression level of T-bet in benzene poisoning group was 1.52±0.56.CONCLUSION: Exposure to benzene affects T cell immune functions.The expression of T-bet and GATA-3 in PBMCs of benzene-exposed and poisoning workers contributes to evaluating the differentiation changes of Th1 and Th2.更多还原