【摘要】 目的:探讨蜂胶对K562细胞增殖、凋亡的影响及其作用机制。方法:体外培养K562细胞,将不同浓度的蜂胶掺入细胞,采用MTT法检测细胞增殖抑制率,流式细胞术(FCM)检测细胞凋亡率,RT-PCR检测细胞中Nup98 mRNA表达水平。Western blotting检测细胞中Nup98蛋白表达水平。结果:2 mg/L、20 mg/L和200mg/L组蜂胶K562细胞的增殖抑制率和凋亡率明显高于对照组,并具有一定的时间和剂量依赖性。高浓度蜂胶作用后,Nup98 mRNA及蛋白表达均明显低于对照组。结论:蜂胶可抑制K562细胞的增殖,诱导其凋亡,其机制可能与下调Nup98的表达有关。更多还原

【Abstract】 AIM: To study the effect of propolis on the proliferation of K562 cells.METHODS: K562 cells were cultured in vitro.Cell proliferation was measured by MTT method.The apoptotic rate was determined by flow cytometry.RT-PCR was applied to detect mRNA expression of Nup98.The protein level of Nup98 was determined by Western blotting.RESULTS: The inhibitory rates of proliferation induced by propolis at the concentrations of 2 mg/L,20 mg/L and 200 mg/L were obviously higher than that in control cells in a time-and dose-dependent manner.The apoptotic rate was increased in a dose-dependent manner.High concentration of propolis down-regulated the expression of Nup98 at mRNA and protein levels.CONCLUSION: Propolis inhibits the proliferation and induces apoptosis in K562 cells.The mechanism may be related with down-regulation of Nup98.更多还原