【摘要】 目的检测SLC30A9(solute carrierfamily 30 member 9)基因在乳腺癌组织中的mRNA表达情况,了解SLC30A9表达与乳腺癌的关系。方法收集30例手术切除的乳腺癌及其相应的癌旁组织、10例正常乳腺组织及20例乳腺良性病变组织作为研究对象。分别采用半定量逆转录-聚合酶链反应(RT-PCR)检测SLC30A9mRNA的表达并分析临床意义。结果在90例乳腺组织标本中,SLC30A9 mRNA均有表达,4种组织平均光密度比值分别为0.443±0.247、0.427±0.253、0.405±0.209、0.547±0.190,分别两两比较差异均无统计学意义(P>0.05)。其中5例患者存在差异性表达,占16.7%(5/30)。5例患者中3例乳腺癌组织SLC30A9的表达比相应的正常乳腺组织低,2例患者在乳腺癌组织中表达上调。乳腺癌组织中SLC30A9mRNA的平均光密度比值在不同年龄、临床分期、淋巴结转移、远处转移和病理类型的组间比较,差异均无统计学意义(P>0.05)。结论 SLC30A9基因在各种乳腺组织中均有表达,但仅在16.7%的病例中出现差异表达,提示该基因可能不是乳腺癌的主导基因,其差异表达原因有待进一步研究。更多还原

【Abstract】 Objective To investigate the expression of gene solute carrier family 30 member 9(SLC30A9) in human breast cancer,and to explore the correlation between expression of SLC30A9 mRNA and breast cancer.Methods 30 cases of surgically removed breast cancer tissue and their adjacent tissue,10 cases of normal breast tissue and 20 cases of benign breast lesion were enrolled in this study.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of SLC30A9 mRNA,and its clinical significances were analyzed.Results All the 90 samples expressed SLC30A9 mRNA.The average optical density ratios of SLC30A9 mRNA in the cancerous tissues,cancer-adjacent tissues,normal breast tissues and benign breast lesion tissues were 0.443±0.247,0.427±0.253,0.405±0.209 and 0.547±0.190 respectively,without significant difference(P>0.05).Five of the 30(16.7%) cancer tissues showed differential expression,among which 3 down-regulated and 2 up-regulated comparing to the normal counterparts.The average optical density rations of SLC30A9 mRNA were not statistically associated with age,clinical stages,lymphonode metastasis,distant metastasis and histopathological type(P>0.05).Conclusion SLC30A9 expresses in a variety of breast tissues but only 16.7% cases are differential expression,suggestion it may not be the dominant gene for carcinogenesis of breast cancer.更多还原