【摘要】 目的研究叶酸对缺氧大鼠神经干细胞(NSCs)增殖及Notch信号通路相关基因mRNA表达的影响。方法无血清培养基原代培养新生大鼠NSCs,细胞分为对照组(Control),缺氧组(Hypoxia),叶酸组(Folate)和叶酸缺乏组(Folate-D)。除Control组外,其余3组缺氧6h。增殖第7日收集细胞,MTT法检测细胞活力,实时荧光定量PCR检测Notch1、Hes1/5、Mash1和Ngn1/2基因mRNA表达。结果 Folate组细胞活力高于Hypoxia组,Folate-D组细胞活力低于其他3组(P<0.05);Folate组Notch1、Hes1/5mRNA表达高于Hypoxia组,Folate-D组该3个基因mRNA表达低于其他3组(P<0.05);Folate组Ngn1/2mRNA表达低于Hypoxia组(P<0.05);Mash1mRNA表达在各组间差别无统计学意义(P>0.05)。结论叶酸能促进缺氧损伤NSCs增殖,上调Notch信号通路中Notch1和Hes1/5基因表达。更多还原

【Abstract】 Objective To investigate the effects of folate on mRNA expression of genes involved in Notch signal pathway of rat neural stem cells(NSCs)after hypoxia.Method Neonatal rat NSCs were cultured in serum-free cell culture.Individual cultures were assigned to one of 4 treatment groups: Control,Hypoxia,folate supplementation(Folate) and folate deficiency(Folate-D).The latter 3 groups were exposed to hypoxia for 6 h on the 3rd day,then be collected on the 7th day.Cell proliferation was assessed by methyl thiazolyl tetrazolium assay.The expression levels of Notch1,Hes1/5,Mash1and Ngn1/2 mRNA were analyzed by real-time PCR.Results NSCs vitality in Folate group was higher than that in Hypoxia group.Proliferation of NSCs was lowest in Folate-D group(P<0.05).Notch1 and Hes1/5 mRNA expression levels in Folate group were higher than those in Hypoxia group(P<0.05).The expression levels of those three genes were lowest in Folate-D group(P<0.05).The expression levels of Ngn1/2 mRNA in Folate group were lower than those in Hypoxia group(P<0.05).There was no statistical significance in expression level of Mash1 mRNA in all groups(P>0.05).Conclusion Supplementation of NSCs with folate after hypoxia may stimulate cells proliferation and the mRNA expression levels of Notch1 and Hes1/5.更多还原