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中国石蒜属种间关系的trnH-psbA序列分析
Analysis of Interspecific Relationships in Lycoris Herb.Based on trnH-psbA Sequence
【摘要】 利用叶绿体基因trnH-psbA序列对石蒜属16种(含1变种)的种间关系进行了分析。结果表明:所测物种的序列全长在546~641bp,GC含量为35.86%~36.41%,其核苷酸变异位点14个,信息位点5个,这些位点可以区分石蒜属物种;供试材料聚为3类,除中国石蒜、忽地笑和玫瑰石蒜的系统位置有所不同外,与经典分类基本一致。叶绿体基因trnH-psbA序列可以有效地鉴别石蒜属物种及分类,是一种有效分子标记。
【Abstract】 The interspecific relationship of 16 species(including 1 variation)in Lycoris was analyzed using trnH-psbA sequences.The results showed that the length of trnH-psbA sequences of the species was 546-641 bp,GC content of them was 35.86%-36.41%.There were 14 variable sites and 5 information sites,which could be used to identify some species of Lycoris.The clustering showed that Lycoris could be classified into 3 clades.The classification was basically consistent with that of morphology except for L.chinensis,L.rosea and L.aurea.This paper suggested that trnH-psbA could be used as a good molecular marker to identify or classify plants of Lycoris.
【Key words】 Lycoris; trnH-psbA sequence; nucleotide variation; interspecific relationship; China;
- 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2011年08期
- 【分类号】S682.29
- 【被引频次】21
- 【下载频次】398