【摘要】 石油烃厌氧生物降解代谢产物的分析对于石油烃厌氧降解机制的研究、功能微生物的筛选以及微生物活动的原位监测具有指示性作用.综述了近年来石油烃厌氧生物降解代谢产物的研究进展.石油烃厌氧降解的初始活化方式主要包括脱氢羟基化、加延胡索酸以及羧化等.其中,加延胡索酸是不同种类的微生物通常采用的代谢方式.同时,将代谢产物按照气体、无机离子和有机酸进行分类,并针对各类物质特别是瞬时性、低浓度的有机酸类产物常采用的分析方法进行归纳.通过实例强调了代谢产物作为潜在生物标记物的应用,并对石油烃厌氧降解代谢产物分析方法的发展提出展望.图3参58更多还原

【Abstract】 Detection of specific metabolites formed during anaerobic biodegradation of petroleum hydrocarbons is of great importance to understand the mechanisms involved,to screen for functional microbes,and to monitor in-situ microbial activities.This paper focus on recent progress in metabolites formed during anaerobic biodegradation of petroleum hydrocarbons.Distinct biochemical activation strategies include dehydrogenation and hydroxylation,fumarate addition and carboxylation.Among them,fumarate addition yielding succinic acid derivatives is likely the most widespread strategy in different microbial phylotypes.Metabolites formed are classified as gaseous,inorganic ions and organic acids.Their analytical methods,especially for transient and low concentrations of organic acids,are summarized.Case studies are presented to emphasize the application of specific metabolites as potential biomarkers,and the prospects for future research on the detection and characterization of signature metabolites resulting from the anaerobic degradation of petroleum hydrocarbons are proposed.Fig 3,Ref 58更多还原