【摘要】 从化工厂污水处理池污泥中分离到一株能高效降解硝基苯的菌株XY-1,通过形态观察、生理生化特征和16SrDNA序列同源性分析,将该菌株鉴定为假单胞菌属(Pseudomonas sp.).该菌株能以硝基苯为唯一碳源、氮源和能源生长,硝基苯初始浓度为200 mg/L时,20 h降解率可达97%.该菌在温度25～35℃、pH 7.0～9.0范围内均能高效降解硝基苯,并且对对氯硝基苯、对氯苯胺也有良好的降解效果.测序分析表明,克隆到了该菌中的硝基苯还原酶基因,推测该菌的降解途径是硝基苯部分还原途径.图6参19更多还原

【Abstract】 A strain designated as XY-1,capable of degrading nitrobenzene efficiently,was isolated from the sludge in an chemical factory wastewater treatment plant.Strain XY-1 was identified preliminarily as Pseudomonas sp.based on its physiological and biochemical characters and the result of 16S rDNA homologue sequence analysis.Strain XY-1 could grow with nitrobenzene as its sole carbon source and nitrogen source,and with the nitrobenzene concentration of 200 mg/L,the degradation rate was 97% within 20 h.The optimal initial pH value and temperature for nitrobenzene degradation by XY-1 was 8.0 and 30 °C,respectively.Strain XY-1 could also degrade some other chemical compounds,such as 4-chloroaniline and 4-chloronitrobenzene.The putative gene cloned was 771 bp long with 99% identical to the gene encoding nitrobenzene nitroreductase,therefore it was presumed that the degradation process was partial reductive pathway.更多还原