【摘要】 石杉科植物因所含石杉碱甲(Huperzine A)对中老年痴呆等具有良好疗效,近年来倍受关注.利用AFLP分子标记对武夷山脉广布种长柄石杉[Huperzia serrata(Thunb.ex Murray)Trev.var.longipetiolata(Spring)H.M.Chang]7个居群112株个体进行遗传多样性和居群遗传结构分析.选用多态性高、分辨力强的8对选择性扩增引物组合共获得675个位点,其中多态位点比例为69.38%.居群内观测等位基因数(Na)为1.633,有效等位基因数(Ne)1.493;Nei’s基因多样性指数(He)与Shannon多态性信息指数(I)的平均值分别为0.272和0.392,多样性最高为地处武夷山脉中段的泰宁居群和建宁居群,最低为山脉北段的光泽居群.居群总基因多样性(Ht)为0.327 3,居群内基因多样性(Hs)为0.272 2,居群间的遗传分化系数(Gst)为0.168 1,表明居群内变异是长柄石杉遗传多样性的主要来源.由Gst估计,武夷山脉长柄石杉自然居群的基因流(Nm)为2.474 0.邻接树分析表明居群间遗传亲缘关系与地理位置相关.武夷山脉长柄石杉较高的遗传多样性和基因流水平表明其仍然具有相当的适应(生存)能力和进化潜力,这可能与其生物学(异交水平)、生态学特性及武夷山脉相对良好的生境条件有关.更多还原

【Abstract】 Huperziaceae has attracted the attention of researchers in recent years because of its Huperzine A(HupA) being a potent,reversible and selective acetylcholinesterase inhibitor(AChEI).The genetic diversity and genetic structure of seven populations and 112 individuals of Huperzia serrata(Thunb.ex Murray) Trev.var.longipetiolata(Spring) H.M.Chang from the Wuyi Mountains were analyzed by amplified fragment length polymorphism(AFLP).We recorded a total of 675 amplified bands using 8 pairs of AFLP primers,555 of which(PPB = 69.38%) were polymorphic.The observed number of alleles(Na) and effective number of alleles(Ne) were 1.633 and 1.493,respectively.The average values of Nei’s genetic diversity index(He) and Shannon polymorphism information index(I) were 0.272 and 0.392,respectively;the Taining and Jianning populations located showed that the genetic sibship among/between populations was correlated with geographical location.This study provides a in the intermediate section of the Wuyi Mountains showed the highest diversity,while the Guangze population distributed in the north section of the Mountains had the lowest diversity.The total genetic diversity(Ht) was 0.327 3,and the population gene diversity(Hs) was 0.272 2.The coefficient of gene differentiation among populations within species(Gst) was 0.168 1,suggesting that intra-population variation is the main source of the genetic diversity of H.serrata var.longipetiolata from the Wuyi Mountains.Estimated from Gst,the gene flow(Nm) of this natural population was 2.474.Adjacent tree analysis(NJ) profound basis for the resource protection of H.serrata var.longipetiolata.更多还原