【摘要】 目的建立测定茵陈合血胶囊中黄芩苷含量的反相高效液相色谱(RP-HPLC)法。方法色谱柱为Shimpak VP-ODS柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-醋酸(52∶48∶1),流速为1.0 mL/min,检测波长为280 nm,进样量为10μL,柱温为25℃。结果黄芩苷与杂质色谱峰分离良好;平均加样回收率为99.96%,RSD=0.23%(n=6);日内、日间精密度的RSD均小于5%,黄芩苷质量浓度在10.55～63.30 mg/L范围内与峰面积线性关系良好,r=0.999 8。结论方法简便、灵敏、快速、准确、重复性好,可用于茵陈合血胶囊中黄芩苷的含量测定和稳定性分析。更多还原

【Abstract】 Objective To establish a HPLC method for the determination of baicalin in Yinchen Hexue Capsule.Methods The separation of sample was carried on the Shimpak VP-ODS column(250 mm×4.6 mm,5 μm) with the mobile phase of methanol-water-acetic acid(52 ∶48 ∶1) at the flow rate of 1.0 mL/min.The detection wavelength was set at 280 nm.The sample size was 10 μL and the column temperature was 25 ℃.Results The chromatographic peaks of baicalin and impurities were separated well.The mean recovery rate of baicalin was 99.96%,RSD=0.23%(n=6).The inter-day RSD and intra-day RSD were all less than 5%.The linearity was determined over the range of 10.55-63.30 mg/L(r=0.999 8).Conclusion This method is simple,sensitive,rapid,accurate and reproducible,which is suitable for the quantitative determination and the stability study of baicalin in Yinchen Hexue Capsule.更多还原