【摘要】 目的建立测定尼美舒利分散片含量的反相高效液相色谱法。方法采用LBondapak C18色谱柱(300 mm×4.0 mm,5μm),乙腈-水(45∶55)为流动相,柱温为25℃,检测波长为254 nm,流速为1.0 mL/min。结果尼美舒利质量浓度在40～200μg/mL范围内与峰面积线性关系良好(r=0.9999),平均回收率为99.60%,RSD为0.47%(n=6)。结论该方法简便、快速、准确、灵敏度高、重复性好,可用于尼美舒利分散片含量的测定。更多还原

【Abstract】 Objective To establish a RP-HPLC method for determining the content of Nimesulide Dispersible Tablets.Methods The LBondapak C18 column(300 mm×4.0 mm,5 μm) was used.The mobile phase was acetonitrile-water(45 ∶55),the temperature was 25 ℃,the detection wavelength was at 254 nm and the flow rate was 1.0 mL/min.Results The linear range of nimesulide was 40-200 μg/mL(r=0.999 9).The average recovery rate was 99.60%,RSD 0.47%(n=6).Conclusion The method is convenient,fast,accurate and sensitive with good reproducibility,which can be used for the content determination of Nimesulide Dispersible Tablet.更多还原