【摘要】 目的探讨缬草提取物对高胆固醇-高脂肪-高糖膳食所致的胆汁-胆汁酸代谢变化及肝损害的影响。方法①大耳白种家兔24只,随机分为3组,每组8只。治疗组和对照组给予高胆固醇-高脂肪-高糖饲料。治疗组每天灌胃缬草提取物30 mg.kg-1,对照组灌胃等容积0.9%氯化钠溶液,正常组自由进食标准颗粒饲料,同时每天灌胃等容积0.9%氯化钠溶液。观察实验兔的血清丙氨酸氨基转移酶(ALT)、血总胆固醇(TC)及总胆汁酸(TBA)含量、胆汁的流量及其TC-磷脂-TBA含量、胆囊结石形成情况、肝组织学检查以及主动脉壁粥样斑块观察。②Wistar大鼠24只,随机分为3组,每组8只。治疗组和对照组给予高胆固醇-高脂肪-高糖饲料,正常组自由进食基础饲料。治疗组每天灌胃缬草提取物50 mg.kg-1,对照组则每天灌胃等容0.9%氯化钠溶液。观察血清TC、TBA及肝组织学检查。结果①家兔治疗组胆总管胆汁流量及固形物含量较对照组显著增加(P<0.01),胆汁中的TBA含量明显增加(P<0.05),血清TBA较对照组明显降低(P<0.05),胆囊内凝结物形成率明显低于对照组(P<0.05)、肝损伤较对照组显著减轻。②大鼠治疗组血清TBA较对照组降低(P<0.05),肝损伤较对照组减轻。结论缬草提取物可以增加胆汁中TBA含量,提高TBA/TC结石形成,降低血清TBA浓度,明显减轻肝损伤。更多还原

【Abstract】 Objective To explore the effects of valerian extracts on bile acid metabolism and liver damage caused by high cholesterol-high sugar-high fat diet. Methods ①24 white rabbits with big ears were randomly divided into 3 groups(n=8 each).The rabbits were fed with high cholesterol-high fat-high sugar diet except those in the normal group with pellet feed.The treatment group was intragastrically given with valeriana extracts 30 mg·kg-1 daily.The serum pyruvate aminotransferase(ALT),serum total cholesterol(TC),total bile acid(TBA) content,bile flow and TC-phospholipids-TBA content,gallstone formation,liver histological test and aortic plaque-like porridge were analyzed later.②24 Wistar rats were randomly divided into 3 groups(n= 8),which were fed with high cholesterol-high fat-high sugar diet,except for those in the normal group with basal diet.The treatment group were fed with valerianaextracts 50 mg·kg-1 daily,the control group was given with the equal volume of 0.9% sodium chloride solution.The serum TC,TBA and liver histology were observed. Results ①Compared with the control group,common bile duct flow and solids content were increased significantly(P<0.01) in the treatment group,TBA concentration in bile was elevated obviously(P<0.05),serum TBA was predominantly lower(P<0.05),gallstone formation was significantly less(P<0.05),liver injury was significantly alleviated.②The serum TBA in rats was dropped(P<0.05) in the treatment group compared with that in the control,and the liver injury was attenuated. Conclusion Valeriana extracts could increase the TBA content of bile,improve TBA/TC stone formation,lower the serum TBA concentration and significantly reduce the liver injury.更多还原