【摘要】 目的探讨替米沙坦对非酒精性脂肪性肝炎大鼠脂联素及PPAR-γmRNA表达的影响。方法 35只雄性SD大鼠随机分为正常对照组(n=10)、模型组(n=15)和替米沙坦干预组(n=10)。模型和药物干预组给予高脂饲料喂养16周诱发脂肪性肝炎,其中药物干预组于高脂喂养12周后,给予替米沙坦(5mg.kg-1.d-1)灌胃治疗4周。检测空腹血糖、空腹胰岛素和胰岛素抵抗指数(HOMA-IR);应用逆转录-聚合酶链反应(RT-PCR)方法测定大鼠肝组织脂联素及PPAR-γmRNA的表达;ELISA法测定血清脂联素水平。结果与正常对照组比较,模型组空腹血糖、空腹胰岛素和HOMA-IR均显著性升高(P<0.01),药物干预组上述指标较模型组显著下降(P<0.01)。与正常对照组比较,模型组血清脂联素及肝组织脂联素、PPAR-γmRNA水平均显著降低(P<0.01);药物干预组的血清脂联素及肝组织脂联素、PPAR-γmRNA较模型组均升高(P<0.05)。结论替米沙坦对非酒精性脂肪性肝炎有防治作用。更多还原

【Abstract】 Objective To explore the effects of telmisartan on expression of adiponectin and PPAR-γ in rats with nonalcoholic steatohepatitis(NASH).Methods Thirty-five male SD rats were randomized into normal model group(NC,n=10),NASH control group(FC,n=15) and telmisartan prevention group(FT,n=10).NC group was given with standard food,the other 2 groups were given with high fat diet for 16 weeks to induce NASH,and FT group was given with telmisartan(5 mg.kg-1.d-1) for 4 weeks by intragastric adminstration 12 weeks later.Fasting blood glucose and insulin were measured and insulin resistance value(HOMA-IR) was assessed by homeostasis mode assessment.Liver expression of adiponectin and PPAR-γmRNA were detected with RT-PCR.The level of plasma adiponectin was detected with ELISA.Results The level of FBG,FINS and HOMA-IR were signficantly higher in group FC than those in group NC(P<0.01),but those in group FT were signifcantly decreased than those in group FC(P<0.01).The level of adiponectin and adiponectin,PPAR-γmRNA were significantly decreased in group FC than those in group NC(P<0.01).The level of adiponectin and adiponectin,PPAR-γmRNA were higher in group FT than those in group FC(P<0.05).Conclusion Telmisartan has the protective and therapeutic effect for nonalcoholic steatohepatitis.更多还原