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藏波罗花中红景天苷的含量测定
Determination of Salidroside in Incarvillea Younghusbandii
【摘要】 目的:测定藏波罗花中红景天苷的含量。方法:采用高效液相色谱法,Diamonsil C18色谱柱,检测波长为223 nm,流动相为乙腈-水(7∶93),流速为1 ml/min。结果:藏波罗花中红景天苷在0.037~1.480μg范围内,线性关系良好(r=0.997 9)。平均回收率为100.5%,RSD=2.3%。测定了6批不同产地的藏波罗花中红景天苷的含量,含量范围为0.181~0.360 mg/g。结论:本法快捷、简便,结果准确、可靠,可作为藏波罗花中红景天苷含量测定的方法。
【Abstract】 Objective:To determine Salidroside in Incarvillea Younghusbandii.Methods:Diamonsil C18(4.6 mm × 250 mm,5 μm) was used with acetonitrile-water(7∶93) as the mobile phase with the flow rate of 1.0 ml/min and UV detection was at 223 nm.Results:The linear range was 0.037-1.480 μg(r=0.997 9),the average recovery was 100.5 % with RSD of 2.3%.6 batches of Incarvillea Younghusbandii were analyzed.The content of Salidroside ranged from 0.181 to 0.360 mg/g.Conclusion:The method is simple,accurate and very suitable for the determination of Salidroside in Incarvillea Younghusbandii.
- 【文献出处】 中国医药导报 ,China Medical Herald , 编辑部邮箱 ,2011年30期
- 【分类号】R284.1
- 【被引频次】3
- 【下载频次】113