【摘要】 目的:采用实时荧光PCR法检测手足口病患儿病原体。方法:收集40例本市确诊手足口病患儿的肛拭子样本,进行肠道病毒筛选和柯萨奇病毒16型与肠道病毒71型鉴别与分析。结果:实时荧光PCR法检测40例患儿肛拭子标本使用通用型试剂盒检出22例阳性,其中9例为CVA-16感染,12例为EV71感染,发病3 d内采集标本检出阳性患儿占75%。结论:CVA-16和EV71肠道病毒是手足口病的主要病原体,手足口病病毒检出率与标本的采集时间相关性,实时荧光PCR技术对手足口病的病原诊断具有重要价值。更多还原

【Abstract】 Objective: To detect pathogens of HFMD by RT-PCR.Methods: The experiment collected anus swab samples of 40 patients for intestinal virus screening.CoxA16 and EV71 identified and analyzed.Results: Swab specimens of patients detected 22 cases by RT-PCR,The CVA-16 tested in 9 patients,the EV71 tested in 12 patients.Specimens within three days detected 75%.Conclusion: CoxA16 and EV71 virus are the main pathogens in HFMD,HFMD virus detection rate is relate with the collection period.RT-PCR technology is important value in HFMD pathogen diagnosis.更多还原