【摘要】 目的:探讨班贞1号和川芎嗪(TMP)联合逆转人胃癌细胞系SGC-7901/ADR对5-FU耐药性与bcl-2表达的相关性。方法:采用半定量逆转录聚合酶链反应(RT-PCR)比较SGC-7901/ADR细胞在单纯5-FU和5-FU联合中药班贞1号及TMP处理组bcl-2 mRNA的表达情况。结果:5-FU组与阴性对照组相比,bcl-2 mRNA表达无显著性差异(P>0.05),而5-FU联合中药班贞1号及TMP处理组bcl-2 mRNA表达量明显降低(P<0.05)。结论:班贞1号和TMP联合逆转人胃癌细胞对5-FU耐药性可能与抗调亡分子bcl-2相关,为中西药联合治疗胃癌奠定一定的理论基础。更多还原

【Abstract】 Objective: To explore of the correlativity between Banzhen No.1 and Tetra methylpyrazine(TMP) reversing human gastric cancer cell line SGC-7901/ADR of 5-FU Drug resistance and bcl-2 expressions.Methods: Semi-quantitative Reverse Transcriptase Polymerase Chain Reaction(RT-PCR) was used for comparison of bcl-2 mRNA expression for cell line SGC-7901/ADR in group of 5-FU、5-Fu and Banzhen No.1,Tetramethylpyrazine(TMP).Results: The expression of bcl-2 mRNA between the 5-FU group and the control group,there was no significant differences(P>0.05),while 5-Fu and Banzhen No.1 group and etramethylpyrazine(TMP) group was decreased significantly(P<0.05).Conclusion: It is possible correlated to Anti-apoptosis Molecularly bcl-2 for Banzhen No.1 and TMP reversed human gastric cancer cells to 5-FU drug resistance,for treatment of gastric cancer with combined Traditional Chinese Medicine and Western Medicine to lay a theoretical foundation.更多还原