【摘要】 目的探讨贵州苗族人群β2肾上腺素受体(β2-AR)基因l6、27位点多态性与支气管哮喘的相关性。方法采用等位基因特异性聚合酶链反应方法,对31例支气管哮喘者、37名健康对照者进行β2-AR基因多态性分析。结果β2-AR基因16位点多态性分布频率在支气管哮喘组为:精氨酸/精氨酸占16.1%、精氨酸/甘氨酸占61.3%,甘氨酸/甘氨酸占22.6%;对照组为精氨酸/精氨酸占13.5%、精氨酸/甘氨酸占70.3%、甘氨酸/甘氨酸占16.2%。27位点多态性分布频率在支气管哮喘组为:谷氨酰胺/谷氨酰胺占32.3%、谷氨酰胺/谷氨酸占54.8%、谷氨酸/谷氨酸占12.9%;对照组中谷氨酰胺/谷氨酰胺占37.8%、谷氨酰胺/谷氨酸占51.4%、谷氨酸/谷氨酸占10.8%,两组在β2-AR基因16、27两位点多态性分布频率比较差异无显著性(P>0.05)。结论贵州苗族支气管哮喘与β2-AR基因16、27位点多态性无关联。更多还原

【Abstract】 Objective To analyze the relationship between β2-drenergic receptor(β2-AR) genetic polymorphism and asthma in the people of the Miao nationality of Guizhou.Methods Allele Specific-PCR techniqu was used to determine 16,27 loci of β2-AR genetic polymorphisms in 31 unrelated patients with asthma,and 37 healthy controls.Results The frequency of genotype β2-AR 16 loci:Arg/Arg genotype accounted for 16.1%,Arg/Gly 61.3% and Gly/Gly 22.6% in asthma group;Arg/Arg for 13.5%,Arg/Gly 70.3% and Gly/Gly 16.2% in control group.The frequency of genotype β2-AR 27 loci:Gln/Gln genotype accounted for 32.3%,Gln/Glu 54.8% and Glu/Glu 12.9% in asthma group;Gln/Gln 37.8%,Gln/Glu 51.4% and Glu/Glu 10.8% in control group.There was no significant diference in the frequencies of genotypes and alleles β2-AR 16,27 loci in both groups.Conclusion β2-AR 16,27 loci genetic polymorphism is not correlated with asthma in the people of the Miao nationality of Guizhou.更多还原