【摘要】 目的评价OPV诱导恒河猴黏膜免疫应答效果。方法将20只恒河猴随机分成3个实验组(第1组:OPV+OPV+OPV,第2组:IPV+IPV+OPV+OPV,第3组:IPV+IPV+OPV)和1个对照组,采集不同时间恒河猴粪便和唾液,检测粪便和唾液中脊髓灰质炎病毒特异性IgA;通过细胞培养和中和试验对口服OPV后恒河猴粪便排毒状况进行分析。结果粪便和唾液中IgA效价随着接种剂数增加而增高,3个组全程免疫后30天粪便样品中IgA效价第1组最高,第2组次之,第3组最低,3组间两两比较有统计学差异(方差分析,P<0.05)。口服OPV后恒河猴粪便中均可分离到3个型的疫苗病毒。结论 OPV可诱导恒河猴咽部和肠道产生良好的黏膜免疫应答,口服OPV后3个型疫苗病毒均能在肠道增殖。更多还原

【Abstract】 Objective To evaluate the mucosal immune effect against poliovirus in Rhesus monkeys induced by OPV.Methods Twenty Rhesus monkeys were divided into 4 groups randomly:group 1(OPV+OPV+OPV),group 2(IPV+IPV+OPV+OPV),group 3(IPV+IPV+OPV),group 4(negative control group).These monkeys′ stool and saliva in different time points were collected to detect 3 types Sabin poliovirus-specific IgA,and the stool sample after immunized with OPV were tested for Sabin strain poliovirus by cell culture and neutralization test.Results The IgA titers of stool and saliva increased with the increasing of OPV dose.Thirty days after the last dose,the highest level of IgA titers was in group 1,then followed by the group 2 and 3.Significant difference existed among the 3 groups(variance analysis,P<0.05).After being immunized with OPV,3 types′ Sabin polioviruses could be isolated from the stool of Rhesus monkeys.Conclusion OPV can induce good mucosal immunity response against poliovirus in Rhesus monkeys.Vaccine viruses of 3 types could be proliferated in Rhesus monkeys′ intestine.更多还原