【摘要】 目的研究黄芩苷对体外铜绿假单胞菌(pseudomonas aeruginosa,Pa)生物膜(biofilm,BF)的影响。方法选取临床分离呼吸道Pa,采用LB肉汤-吸痰管系统培养BF,建立体外BF模型,银染法观察BF变化。将黄芩苷作用于BF,连续稀释法进行活菌计数,微量稀释法测定最低抑菌浓度(MIC)。结果 15.65mg/L的黄芩苷即可抑制和破坏BF。结论黄芩苷对体外铜绿假单胞菌生物膜有较强的抑制作用。更多还原

【Abstract】 Objective To investigate the effect of baicalin on Pseudomonas aeruginosa biofilms in vitro.Methods Clinical isolates of Pseudomonas aeruginosa were cultured in Luria-Bertani medium-aspirate sputum pipe system to establish biofilm formation.Biofilm was observed in AgNO3 staining.Viable bacterial counts were determined by serial dilution.MICs were measured by doubling dilution.Results The biofilm was inhibited and destructed by 15.65mg/L of baicalin.Conclusion Baicalin displayed potent activity against Pseudomonas aeruginosa biofilms.更多还原