【摘要】 [目的]了解环泰山区域恙虫病东方体(Ot)流行株的基因型、遗传特征,探讨人、鼠间所感染Ot的基因同源性和系统进化情况。[方法]2006~2008年对在环泰山区域采集的鼠类和疑似恙虫病病人标本进行Ot-Sta56kDa基因片段检测,对阳性标本进行序列测定;对所测到的序列与GenBank中部分Ot参考序列进行序列同源性分析,绘制系统发生树。[结果]检测84份鼠脾标本,4份Ot基因阳性,阳性率为4.76%。其中,家鼠阳性率为1.79%,黑线姬鼠阳性率为10.71%(P>0.05)。4份病人焦痂标本3份Ot基因阳性。序列同源性分析显示,鼠脾标本08P、53P的核苷酸序列与Sdu-1型同源性为99.77%,与Taguehi和Oishi型的同源性均为95.98%,与Kanda和Kawasaki型的同源性均为95.74%;鼠类标本55P、56P的核苷酸序列与TA686型的同源性分别为86.27%和77.11%。3份病人阳性标本的核苷酸序列与鼠标本08P、53P同源性均为100.00%,与55P、56P的同源性仅为74.30%。系统发生树分析显示,08P、53P和3份病人阳性标本与Sdu-1型在同一分支,与Kawasaki、Kanda、Tagnchi和Oishi型共处于同一分支;55P和56P与TA686型在同一分支。[结论]环泰山区域人、鼠间均存在Ot自然感染,病人Ot与部分鼠类感染Ot基因同源,Ot的基因型较为复杂。更多还原

【Abstract】 [Objective]To investigate the genotype,genetic homology and phylogeny of the Ot infecting human and rodents in the area surrouding Mount Tai.[Methods]The Ot-Sta56KDa gene of patients eschar collected and rodents captured from the area surrouding Mount Tai was identified by nested polymerase chain reaction(nPCR) in 2006-2008.Positive samples were sequenced,and the DNA sequences were compared with nucleotidesequences of Ot registered in GenBank for sequence homology analysis,Phylogenetic analysis about the Ot in Mount Tai and some published sequences was carried out with Neighbor-joining method by MEGA3.1 software.[Results]84 rodents spleen samples were detected,there were 4 samples positive(1 Mus musculus and 3 Apodemus agrarius),the positive rate was 4.76%.Among them,the positive rate was 1.79 % in Mus musculus,and Apodemus positive rate was 10.71%(corrected chi-square test(P>0.05).A total of 4 patients eschar collected,there were 3 positive.Comparing with the sequences of Ot registered in GenBank,the Ot-Sta56 nucleotide sequences of 08P,53P and 3 patients eschar samples both shared 99.77% of homology with Sdu-1 strain,95.98% with Taguchi and 0ishi strains,and 95.74% with Kanda and Kawasaki strains.In addition,they shared 100.00%.The nucleotide sequence of 55 P shared 86.27% of homology with TA686 strain founded in Thailand.and the sequence of 56P shared 77.11% homology with TA686 strain.Phylogenetie analysis showed that 08P,53P and 3 patients eschar were in the same branch with Sdu-1 strain.and 55P and 56P were in the same branch with TA686 strain.[Conclusion]This study indicated that Ot infection was presented in human and rodents from Taishan area,and the Ot that the patients and some rodents infected were completely homologous gens,so,the natural foci of scrub typhus has been formed.It was here that The genotypes of Ot were complex.更多还原