【摘要】 探讨重组水蛭素Ⅲ(rHV3)对半乳糖损伤的人晶状体上皮细胞(hLEC)热应激蛋白(Hsp)表达的影响。采用125×10-3mol/LD-半乳糖建立人晶状体上皮细胞SAR01/04损伤模型,用重组水蛭素Ⅲ干预受损细胞,设置正常组、模型组、和重组水蛭素Ⅲ组,用倒置显微镜观察各组细胞密度和形态,RT-PCR检测人晶状体上皮细胞内3种热应激蛋白mRNA的表达。结果显示,125×10-3mol/LD-半乳糖导致人晶状体上皮细胞显著损伤,3种热应激蛋白,即Hsp70、Hsp27和αB-晶体蛋白的mRNA表达显著上调。重组水蛭素Ⅲ的干预使细胞形态趋于正常,细胞内Hsp70、Hsp27和αB晶体蛋白的mRNA表达显著下调。因此,重组水蛭素Ⅲ能有效缓解高浓度半乳糖导致的热应激蛋白应激耐受,从而保护晶状体上皮细胞。更多还原

【Abstract】 The aim of the paper is to investigate the effects of recombinant hirudin variant Ⅲ(rHV3) on the expression of heat shock proteins in human lens epithelial cell(hLEC) line SAR01/04 damaged by galactose.Damaged hLEC model was established using 125×10-3mol/L D-galactose,and rHV3 was used to interfere the damaged cells.There were three experimental groups: normal group,model group,and rHV3 group.The cells were observed under the phase-contrast microscope for any morphological changes.The expression of three heat shock proteins at the mRNA levels in cells was detected using RT-PCR.The cells were significantly damaged,and the mRNA levels of Hsp70、 Hsp27 and αB-crystallin were upregulated,under the condition of 125×10-3mol/L D-galactose.rHV3 prevented the cellular morphological changes and elevation of Hsp70、 Hsp27 and αB-crystallin mRNAs.These results indicated that rHV3 could attenuate stress tolerance of heat shock proteins insulted by D-galactose and have the positive function in protecting the hLEC.更多还原