【摘要】 目的:建立肠炎清合剂中君药黄连的鉴别及盐酸小檗碱、盐酸巴马汀含量测定方法。方法:采用薄层色谱法对合剂中黄连进行定性鉴别;采用HPLC进行含量测定。结果:薄层色谱均能明显的检出黄连的特征有效成分盐酸小檗碱和盐酸巴马汀;HPLC法中,盐酸小檗碱在1.34～42.8μg.mL-1内线性关系良好(r=0.9999),平均回收率99.3%,RSD=0.92%(n=9);盐酸巴马汀在1.24～39.8μg.mL-1内线性关系良好(r=0.9999),平均回收率98.6%,RSD=0.82%(n=9)。结论:本法简便、快速、灵敏、准确,重现性好,可以作为该合剂的质量控制方法。更多还原

【Abstract】 Objective:To establish a method for identification of Rhizoma Coptidis in Changyanqing mixture by thin-layer chromatography(TLC)and determination of berberine hydrochloride and palmatine hydrochloride by high performance liquid chromatography(HPLC).Methods:Identification of palmatine hydrochloride and berberine hydrochloride as the effective constituent of Rhizoma Coptidis in preparation by TLC and determination these two components by RP-HPLC.Results:Both characteristics of the active ingredient of Rhizoma Coptidis could be identified by TLC.The linear range of berberine hydrochloride was within 1.34-42.8 μg·mL-1(r=0.9999),the mean recovery was 99.3% with RSD=0.92%(n=9).The linear range of palmatine hydrochloride was within 1.24-39.8 μg·mL-1(r=0.9999),the mean recovery was 98.6% with RSD=0.82%(n=9).Conclusion:The method is simple,rapid,sensitive and accurate with good repeatability,it can be used as the quality control method for Changyanqing mixture.更多还原