【摘要】 目的:建立HPLC法同时测定半合成紫杉醇中的10-去乙酰巴卡亭Ⅲ、巴卡亭Ⅲ、7-三氯乙氧羰酰巴卡亭Ⅲ、13-O-[(4S,5R)-2-(4-甲氧基苯基)-N-苯甲酰基-4-苯基-噁唑烷基]-7-三氯乙氧羰酰巴卡亭Ⅲ和紫杉醇的含量。方法:用Angilent ODS C18分析柱(4.6 mm×150 mm,5μm),甲醇-乙腈-0.1%乙酸水溶液线性梯度洗脱:0～8.5 min(24:31:45),8.5～38.5 min(57:43:0),38.5～60 min(24:31:45);流速0.8 mL.min-1,检测波长227 nm。结果:该测定方法回收率高,成分的分离度好。结论:该法简便快速,重现性好,适用于半合成紫杉醇的质量控制。更多还原

【Abstract】 Objective:To establish an HPLC method for determination of 10-deacetyl baccatin Ⅲ,baccatin Ⅲ,7-trichloroacetyl-baccatin Ⅲ,13-O-[(4S,5R)-2-(4-methoxyphenyl)-N-benzoyl-4-phenyl-oxazolidyl]-7-trichloroacetyl-baccatin Ⅲ,paclitaxel form semisynthetic paclitaxel.Method:Angilent ODS C18 4.6 mm×150 mm,5 μm)column was used.The mobile phase of methanol-acetonitrile-0.1% acetic acid water solution 0-8.5 min(24∶31∶45),8.5-38.5 min(57∶43∶0),38.5-60 min(24∶31∶45) was pumped at a flow rate of 0.8 mL·min-1 and detection wavelength of 227 nm.Result:Recovery and separation of the method was very well.Conclusion:This method is simple,quick,reproducible and is suitable for the quality control of semisynthetic paclitaxel.更多还原