【摘要】 目的探讨MCP-1A-2518G基因多态性在2型糖尿病的发生以及其血管并发症的发展中的作用。方法选择健康对照组55例,2型糖尿病患者135例,所有入选对象留取空腹静脉血,提取DNA,PCR扩增、酶切分析其基因型,应用病例-对照研究方法探讨MCP-1基因多态性与2型糖尿病及其并发症的关系。结果 (1)2型糖尿病患者MCP-1GG(-2518)基因型频率及G(-2518)等位基因频率显著高于正常对照组(P<0.01),差异有统计学意义。(2)2型糖尿病无血管并发症组与合并血管并发症组之间GG基因型及G等位基因频率差异无统计学意义(P>0.05)。结论在中国昆明汉族2型糖尿病人群中,MCP-1G(-2518)等位基因频率明显增高,MCP-1G(-2518)等位基因可能是2型糖尿病发生的危险因素,MCP-1A-2518-G基因多态性可能与2型糖尿病的发生有一定的相关性。更多还原

【Abstract】 Objective To approach the role of polymorphism of MCP-1 genes in the pathogenesis and development of type 2 diabetes mellitus(T2DM)and its macrovascular and microvascular complications.Methods We genotyped 55 normal subjects and 135 type 2 diabetes patients.All the subjects were drawed of fasting venous blood,then extracted genomic DNA.Genotypes of the MCP-1G(2518)A were performed by polymerase chain reaction followed by digestion with appropriate restriction endonucleases.The results of determination were analyzed statistically.Results(1) The frequencies of the GG(-2518) genotype and the frequencies of the MCP-1 G(-2518) allete were significantly higher in patients with T2DM compared with normal controls,and there was statistical significance(P<0.01).(2) There were no statistical significances for frequencies of the GG(-2518) genotype and the frequencies of the MCP-1 G(-2518) allete with diabetic complications compared to the uncomplicated(P>0.05).Conclusion The frequencies of MCP-1 G(-2518) allele were significantly higher in the Han population of type 2 diabetes in Kunming,China.MCP-1 G(-2518) allete may be the risk factor of T2DM,and polymorphisms of MCP-1 genes may be associated with T2DM.更多还原