【摘要】 分别用3种前处理方法对金银花中的绿原酸进行提取,用高效液相色谱法进行检测.结果表明,用匀浆法提取所需时间明显少于热回流提取法和超声波提取法,采用80%的甲醇匀浆法提取金银花中的绿原酸,提取液中的绿原酸以W aters X terra RP18(3.9 mm×150 mm,5μm)色谱柱为固定相,1%醋酸为流动相,梯度洗脱,流速为0.8 mL/m in,检测波长345 nm.在该色谱条件下,绿原酸质量浓度在5.0~200μg/mL内成良好的线性关系,样品平均加标回收率为118%,相对标准偏差(RSD)为4.5%(n=6),结果令人满意.更多还原

【Abstract】 In this paper,three sample preparation for determination of chlorogenic acid in flos lonicerae was studied.Test results showed that the time spent on high-speed homogenization extraction was obviously less than the ultrasonic extraction and the hot reflux extraction.The chlorogenic acid was extracted from samples by high-speed homogenization with 80% methanol solution.The extract was separated on a Waters Xterra RP18 column(3.9mm×150 mm,5 μm) using 1% acetic acid as the mobile phase with gradient elution,the flow rate was 0.8 mL/min.The detection wavelength was 345 nm.Under this condition,the linear of chlorogenic acid was in the range of 5.0—200 μg/mL.The average recoveries of the samples were 118%,and the relative standard deviation was 4.5%(n=6).This method for the extraction and determination of chlorogenic acid in flos lonicerae has good results.更多还原