【摘要】 从50对AFLP引物中筛选出9对多态性高、扩增稳定、重复性好的引物,对敦化一化型、洮南二化型和公主岭二化型3个地理种群共24个个体进行遗传多样性分析,研究不同化性亚洲玉米螟间的遗传关系。结果表明,9对引物共扩增出1 127条清晰可辨的DNA条带,其中多态性条带1 029条,平均多态性比例为91.30%;亚洲玉米螟不同种群个体间遗传相似性指数分布在0.430 7～0.667 5之间;同一种群内个体间的遗传相似性指数敦化一化型为0.439 9～0.524 0,洮南二化型为0.487 8～0.667 5,公主岭二化型为0.459 7～0.563 9,遗传相似性较低,表明亚洲玉米螟遗传多样性十分丰富。聚类分析结果表明,供试的24个亚洲玉米螟聚成两大类,敦化一化型聚为一类,洮南二化型和公主岭二化型聚为一类。更多还原

【Abstract】 For further understanding the population dynamics and improving the forecast and control of this species,the DNA polymorphism of 3 geographic populations and different voltinism types was analyzed by using amplified fragment length polymorphism(AFLP) technique.The results showed that 1 127 bands were amplified with 9 pairs of primers and 1 029 polymorphic loci were found,which accounted for 91.30%.Genetic similarity coefficients of Asian corn borer individuals were changed from 0.430 7 to 0.667 5.By cluster analysis,twenty four accessions were grouped into two groups,Dunhua univoltine population is clustered in one group,Gongzhuling bivoltine and Taonan bivoltine populations are clustered in another group.更多还原